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Study conducted by Dr. Ronald J. Riegel D.V.M.
Harness racing is one of the most demanding of all of the equine athletic endeavors. Months of foundation miles are accomplished before this athlete is even close to race condition and speed. After this long period of training, the animal must then drop in time, over the mile distance, to be competitive. This hard training regime is extremely taxing upon the equine athlete and all methods must be considered to alleviate the pain and inflammation within the musculature to ensure the success of this equine athlete.
The Thermotex™ Infrared heating blanket has been scientifically proven to be effective in providing deep heat to the musculature of the equine athlete. Infrared thermographs correlated with blood chemistry analysis will provide proof that this physical therapy modality is effective in relieving both pain and inflammation within the muscle tissues of these equine athletes.
An infrared thermograph is a pictorial photograph of the surface temperature of the anatomical area it is measuring. The circulatory system, inflammation, environmental changes, the metabolic rate of the animal and other individual thermographic characteristics influences its measurements.
The use of infrared thermography as a measurement of the efficacy of different therapeutic modalities is easily accomplished. Changes within thermal gradients can depict a decrease or increase in circulation, a decrease or increase in inflammation and even nerve irritation somewhere along the neuron pathway. Therapeutic results can be visualized by a series of infrared thermographs taken over a period of time and then compared.
These are the questions that will be answered by this research endeavor.
Racing equine have track records that are public information. Typically, as with their human athlete counterparts, the presence of injury causes a reduction in their performance. When treated with Thermotex™ (controlling for pathologies and no other clinical intervention) the animals returned to their original track records. Unlike their human counterparts, horses have limited cognitive abilities. Therefore, it would be very difficult to argue any precognition or anticipation of outcome of a clinical intervention on the part of the horse.
AST is an abbreviation for Aspartate aminotransferase which is the synonym for the old term SGOT (serum glutamic oxaloacetic transaminase). This enzyme occurs in almost all cells within the body but it is used to primarily diagnose liver and muscle disease. The liver and muscle cells have the highest activity of this enzyme. In itself it is not specific for a liver disorder but is more diagnostic for the muscle tissues.
Aspartate aminotransferase is present in the mitochondria and the cytoplasmic fluid within the cells. The serum levels of this enzyme are increased following hard exercise or skeletal muscle injury. Circulating concentrations of this enzyme will peak approximately 24 hours after an inciting incident and return to normal within 7-10 days.
CPK (CK) is an abbreviation for Creatine phosphokinase (or Creatine kinase). This is the most organ specific of all of the clinical enzymes. Most serum CPK activity is from a muscular origin. The plasma half-life of this enzyme is short and will peak as early as six hours. This enzyme will then only take 2-3 days to return to normal.
Two groups of ten three and four-year-old standardbred racehorses that are in full training were utilized as the test subjects. These twenty animals will have to meet the following criteria:
The study will last six weeks, have a two-week break to all equine subjects and then resume with the control group becoming the treatment group and the treatment group becoming the control group for another six weeks.
All twenty animals will be thermographed initially, and then at weekly intervals during the duration of the study. Blood samples will be taken initially from all of the animals and then repeated weekly until the conclusion of the study. These blood samples will be evaluated for a complete blood count and total serum chemistry analysis.
The complete blood count will have the following parameters tested:
The animals will randomly placed into two groups: a treatment group and a control group.
Those animals within the control group will not receive any treatments with the Thermotex™ therapy blanket during that portion of the study. The animals within the treatment group will receive treatment for thirty minutes each day just before exercise. The temperature control was placed on the high setting for ten minutes and then on low for the remaining twenty minute duration of the treatment.
Infrared thermographs and blood samples were taken every seven days for a total of fourteen weeks.
The initial infrared thermograph (See initial thermograph - animal #5) revealed an increased thermal gradient over the thoracic, lumbar and lumbosacral spine. This thermograph was taken at a setting of 0.5 degrees centigrade per isothermic level. White is the highest reading and purple is the coldest or lowest level with a five-degree difference between them. These areas depicted by the color white were fairly symmetrical except within the lumbosacral area. In this anatomical area, there is an increased thermal gradient predominantly on the left side. These areas of white, gold and yellow reveal an increased thermal gradient within the tissues that is indicative of an inflammatory response within.
This particular animal (#5) was chosen as an example of the entire study since the response within this animal was close to the average of animals within the entire study. This animal also palpated digitally with a slight tenderness throughout the areas that are depicted by the color white.
After eight weeks of training without any treatment, the infrared thermograph of animal number five revealed the following comparisons to the initial thermograph. (See infrared thermograph - animal #5 - week 8) There is an increase within all of the thermal gradients as compared to the initial findings. The increased thermal gradients throughout the thoracic spine now continue into the right shoulder region. Those increased thermal gradients within the lumbar and lumbosacral areas have increased in intensity and are now more predominant on the left side continuing into the gluteal areas. These increases are due to a rigorous training schedule and are normal for an animal that is receiving no therapeutic help for this inflammation.
All ten of the animals that were in the control group depicted these types of thermographs for the first eight weeks of the study. They were all becoming increasingly sore and their thermographs all revealed increases along the lumbar and sacral spine.
Immediately after treatment with the Thermotex™ therapy blanket, there was a huge increase within the thermal gradients within the tissues. This increase lasted an average of four to five hours after the treatment was concluded. These thermographs were done before the initiation of the study just to establish a baseline of data. During the study, the animals were always exercised after their treatment, which also increased the thermal gradients found within the individual making the duration of effect impossible to measure.
The infrared thermograph taken of animal #5 at the conclusion of the study revealed large decreases within the thermal gradients along all of the anatomical areas examined. (See infrared thermograph - week 14) Within the lumbosacral spine area there is a remarkable 95% reduction within the thermal gradients. Clinically, this also corresponds to insensitivity upon digital palpation. The thoracic and thoracolumbar spinal areas also experienced a reduction within the thermal gradients to an extent of a 68% reduction. This area was also no longer sensitive to digital palpation.
The AST levels for the ten animals that served in the control group were consistently high during the first eight weeks of the study and then gradually dropped to lower levels during the last six weeks of the study.
(See table one data, graph and chart of averages)
Initially, seven of the ten animals tested with higher than normal AST levels within the serum. By week eight, eight of the ten animals depicted AST levels higher than normal. After the initiation of treatment at week eight, these levels gradually fell, in all but those animals testing normally, to a level lower than that found at the eight week tests. By week fourteen, six of the ten animals were now testing within normal limits. The results seen within chart one summarize this data.
Those animals that initially received treatment for six weeks and then were left untreated revealed quite different results within their AST levels.
(See table two data, graph and chart of averages)
Five of these ten animals depicted higher than normal AST levels at the initiation of treatment. Nine of the ten animals experienced an immediate drop within the first week of treatment. By week six, seven of the ten animals were testing within normal AST ranges. After the cessation of treatments, seven of the ten animals within this group immediately experienced a rise within the AST levels during week seven. At fourteen weeks, eight of the ten animals revealed higher AST levels than those measured initially. The data graphed on chart two reveals this decline in AST levels through week six and then a gradual increase when the use of Thermotex™ blanket was stopped.
The CK levels followed the same pattern as the AST levels within the group of ten animals that was used as a control for eight weeks and then provided treatment.
(See table three data, graph and chart)
Initially, five of the ten animals tested within the normal limits for CK levels in the horse. By week eight, six of the animals tested with an increase in CK levels over those initial levels. Over the next six weeks, during treatment with the Thermotex™ therapy blanket, all ten of the animals experienced a decline in CK levels. Eight of the ten animals are now testing within normal limits. The other two animals could even be considered in the high normal range.
Chart three is a summary of all of the averages on a weekly basis. During the control part of the study, the group maintained an even level throughout the first 6-8 weeks. After the initiation of treatment, the CK levels gradually fell.
When the animals initially received treatment and then became the control group, the results mimicked those of the same AST group.
(See table four data, graph and chart)
Initially, only four of the ten animals tested within normal limits for CK. After six weeks of treatment with the Thermotex™ therapy blanket, six of the ten animals were within normal limits. By the time fourteen weeks had passed, Seven of the ten animals exhibited higher than normal levels with seven of the ten animals also showing increased levels from the initial testing. Chart four exhibits the gradual decline in CK levels and then the gradual increase after treatment protocols have ceased.
The summary of the blood chemistry analysis revealed that the AST and CK levels were lower during treatment with the Thermotex™ therapy blanket.
(See table five data and graph)
As depicted on the data table and corresponding graph, the AST and CK levels increased while the animals were in training and not receiving treatment with the Thermotex™ therapy blanket and decreased when receiving treatment.
The information on this page was copied with permission from the Thermotex website http://www.thermotex.com/equine/research.htm